Coding
Part:BBa_K3582028:Design
Designed by: Saillesh Aravindhan C, Chinmay Patwardhan Group: iGEM20_IISER-Pune-India (2020-10-24)
Inhibitory sequence 2 for PfEMP1-ICAM1 interaction grafted into a cyclotide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 112
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 143
Illegal BsaI.rc site found at 109
Design Notes
- 1] RFC10 criteria satisfied
- 2] Strep Ⅱ tag gene inserted in loop 3
- 3] Codon optimized with E-coli K-12 strain
- 4] Inhibitor 2 grafted in loop 6
- 5] C and N intein added for circularization
Source
Reverse translation from the amino acid sequence